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guide rna expression plasmid lenti crispr v2  (Addgene inc)


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    Structured Review

    Addgene inc guide rna expression plasmid lenti crispr v2
    Guide Rna Expression Plasmid Lenti Crispr V2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna+expression+plasmid+lenti+crispr+v2/lentiCRISPR+v2+(Plasmid+%2352961)/pmc12456019-240-28-33
    Average 96 stars, based on 6143 article reviews
    guide rna expression plasmid lenti crispr v2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Sequencing:

    Article Title: Heat shock protein A1L restricts influenza A virus by ubiquitination of NA
    Article Snippet: .. The HSPA1L gene target sequence (5′- AATCGCCATAGGCATCGACC -3′), NBR1 gene target sequence (5′- CTGATCCAGAAAATACAACT -3′), and TAX1BP1 gene target sequence (5′- TTACCTTCCTAATGCACACC -3′) were separately cloned into the guide RNA expression plasmid Lenti-CRISPR-V2 (Addgene, 52961, depositing lab: Feng Zhang from Broad Institute). .. The resultant recombinant lentiviral plasmids, along with psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) packaging plasmids, were co-transfected into HEK293KT cells using Polyethylenimine HCl MAX, Linear (P4000, LABLEAD) for 48 h. After centrifugation at 10,000 × g for 10 min at 4°C, the supernatants were added to HEK293T cells.

    Clone Assay:

    Article Title: Heat shock protein A1L restricts influenza A virus by ubiquitination of NA
    Article Snippet: .. The HSPA1L gene target sequence (5′- AATCGCCATAGGCATCGACC -3′), NBR1 gene target sequence (5′- CTGATCCAGAAAATACAACT -3′), and TAX1BP1 gene target sequence (5′- TTACCTTCCTAATGCACACC -3′) were separately cloned into the guide RNA expression plasmid Lenti-CRISPR-V2 (Addgene, 52961, depositing lab: Feng Zhang from Broad Institute). .. The resultant recombinant lentiviral plasmids, along with psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) packaging plasmids, were co-transfected into HEK293KT cells using Polyethylenimine HCl MAX, Linear (P4000, LABLEAD) for 48 h. After centrifugation at 10,000 × g for 10 min at 4°C, the supernatants were added to HEK293T cells.

    RNA Expression:

    Article Title: Heat shock protein A1L restricts influenza A virus by ubiquitination of NA
    Article Snippet: .. The HSPA1L gene target sequence (5′- AATCGCCATAGGCATCGACC -3′), NBR1 gene target sequence (5′- CTGATCCAGAAAATACAACT -3′), and TAX1BP1 gene target sequence (5′- TTACCTTCCTAATGCACACC -3′) were separately cloned into the guide RNA expression plasmid Lenti-CRISPR-V2 (Addgene, 52961, depositing lab: Feng Zhang from Broad Institute). .. The resultant recombinant lentiviral plasmids, along with psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) packaging plasmids, were co-transfected into HEK293KT cells using Polyethylenimine HCl MAX, Linear (P4000, LABLEAD) for 48 h. After centrifugation at 10,000 × g for 10 min at 4°C, the supernatants were added to HEK293T cells.

    Plasmid Preparation:

    Article Title: Heat shock protein A1L restricts influenza A virus by ubiquitination of NA
    Article Snippet: .. The HSPA1L gene target sequence (5′- AATCGCCATAGGCATCGACC -3′), NBR1 gene target sequence (5′- CTGATCCAGAAAATACAACT -3′), and TAX1BP1 gene target sequence (5′- TTACCTTCCTAATGCACACC -3′) were separately cloned into the guide RNA expression plasmid Lenti-CRISPR-V2 (Addgene, 52961, depositing lab: Feng Zhang from Broad Institute). .. The resultant recombinant lentiviral plasmids, along with psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) packaging plasmids, were co-transfected into HEK293KT cells using Polyethylenimine HCl MAX, Linear (P4000, LABLEAD) for 48 h. After centrifugation at 10,000 × g for 10 min at 4°C, the supernatants were added to HEK293T cells.



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    Image Search Results


    Journal: iScience

    Article Title: Targeting ROR2 homooligomerization disrupts ROR2-dependent signaling and suppresses stem-like cell properties of human breast adenocarcinoma

    doi: 10.1016/j.isci.2024.111589

    Figure Lengend Snippet:

    Article Snippet: SpCas9 and chimeric guide RNA expression plasmid lenti-CRISPR v2 vector (Addgene) were used to generate stable ROR2 knockout cell-lines.

    Techniques: Virus, Formalin-fixed Paraffin-Embedded, Recombinant, Mutagenesis, Cloning, Activation Assay, Gene Expression, Sequencing, Plasmid Preparation, Software, Imaging